Composite
Part:BBa_K3844006:Design
Designed by: Anya Sabhnani Group: iGEM21_DNHS_SanDiego_CA (2022-10-11)
Oxybenzone Sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 352
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 425
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added Pribnow Box and a transcription control element to optimize the expression of Emerald GFP to E. Coli.
Source
Plasmid Backbone: pRSET T7 Expression Vector