Composite

Part:BBa_K3844006:Design

Designed by: Anya Sabhnani   Group: iGEM21_DNHS_SanDiego_CA   (2022-10-11)


Oxybenzone Sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 352
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 425
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added Pribnow Box and a transcription control element to optimize the expression of Emerald GFP to E. Coli.


Source

Plasmid Backbone: pRSET T7 Expression Vector

References