Part:BBa_K3841091

Expression cassette of soy leghemoglobin from regulated promoter

BBa_K3841090 is an expression cassette designed for Komagataella phaffii GS115 (previously referred to as Pichia pastoris). The soy leghemoglobin and TEF terminator are codon-optimized for K. phaffii under the control of the FLD1 promoter.

Usage
For chromosomal integration, flank the expression cassette by 65bp long homology arms. The homology arms should match upstream and downstream to an induced DSB. Visit 2021 DTU-Denmark’s Toolbox for K. phaffii to find homology arms. Insert the fragment into a vector backbone of choice for instance the pGGAselect destionation plasmid (BBa_K3841023).

The FLD1 promoter will induce leghemoglobin production on presence of methylamine or methanol.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 143
  Illegal EcoRI site found at 476
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 143
  Illegal EcoRI site found at 476
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 143
  Illegal EcoRI site found at 476
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 143
  Illegal EcoRI site found at 476
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 143
  Illegal EcoRI site found at 476
  
• 1000
  COMPATIBLE WITH RFC[1000]