DNA

Part:BBa_K3841035:Design

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-10-16)


Repair template for ADH2 knockout


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The repair template was made by fusing the 45 base pairs upstream of the AOX2 gene with the 45 base pairs downstream of the of the ADH2 gene.


Source

The DNA fusion construct is complementary to the upstream region of the adh2 gene on chromosome 2 (position 875148-875192) and the downstream region (position 876246-876290) within the assembled genome of K. phaffii GS115 strain by De Schutter et al. 2009 (Accession number FN392320).

References