Part:BBa_K3841023

pGGAselect Destination Plasmid

pGGAselect destination plasmid is a 2,220 bp cloning vector useful for Golden Gate Assembly desgined by New England Biolabs (NEB) [1]. The plasmid contains two BsaI, BsmBI and BbsI restriction sites; digestion with BsmBI releases an 87 bp fragment and a 2,133 bp vector backbone fragment to receive your insert or assembly. Below is the pGGAselect plasmid map from NEB Golden Gate Assembly Kit (BsaI-H®v2) protocol.

Plasmid map of pGGAselect destination plasmid. All features are seen and can be seen in the seuqnece below.

Usage
pGGAselect is suitable for Golden Gate Assembly. Either digest the backbone with BsaI or BsmBI for prepare it for insert or assembly.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 296
  Illegal EcoRI site found at 434
  Illegal PstI site found at 234
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 296
  Illegal EcoRI site found at 434
  Illegal PstI site found at 234
  Illegal NotI site found at 445
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 296
  Illegal EcoRI site found at 434
  Illegal BglII site found at 356
  Illegal BamHI site found at 290
  Illegal XhoI site found at 284
  Illegal XhoI site found at 439
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 296
  Illegal EcoRI site found at 434
  Illegal PstI site found at 234
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 296
  Illegal EcoRI site found at 434
  Illegal PstI site found at 234
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 395
  Illegal BsaI.rc site found at 320
  

References

[1] New England Biolabs, url: https://international.neb.com/-/media/nebus/page-images/tools-and-resources/interactive-tools/dna-sequences-and-maps/text-documents/pggaselectfsa.txt?rev=87941bf93a1d49f28c69930c8289e15f&hash=4A71E1E81374F863510F52544BA65704