Coding
Part:BBa_K3841015:Design
Designed by: Emil Ørsted Christensen Group: iGEM21_DTU-Denmark (2021-09-27)
Particulate methane monooxygenase alpha subunit (PmoB) fused to Venus
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 619
Illegal XbaI site found at 1153
Illegal PstI site found at 1474 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1474
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 334
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 619
Illegal XbaI site found at 1153
Illegal PstI site found at 1474 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 619
Illegal XbaI site found at 1153
Illegal PstI site found at 1474 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used GeneWiz to codon optimize the sequence for expression in Komagataella phaffii. Additionally, we removed any BsaI and BsmBI sites within the coding sequence.
Source
Codon optimized pMMO from https://www.uniprot.org/uniprot/G1UBD1 & Codon optimized Venus from https://parts.igem.org/Part:BBa_K1907000