Coding
Part:BBa_K3841015
Designed by: Emil Ørsted Christensen Group: iGEM21_DTU-Denmark (2021-09-27)
Particulate methane monooxygenase alpha subunit (PmoB) fused to Venus
Particulate methane monooxygenase alpha subunit B (BBa_K3841010) fused with the flourescent protein Venus (BBa_K3841006) via a flexible linker (Gly-Gly-Ser-Gly-Gly-Gly-Gly-Ser-Gly-Gly) not to impair activity [1].
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 619
Illegal XbaI site found at 1153
Illegal PstI site found at 1474 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1474
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 334
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 619
Illegal XbaI site found at 1153
Illegal PstI site found at 1474 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 619
Illegal XbaI site found at 1153
Illegal PstI site found at 1474 - 1000COMPATIBLE WITH RFC[1000]
References
[1] Chen, Xiaoying, et al. “Fusion Protein Linkers: Property, Design and Functionality.†Advanced Drug Delivery Reviews, vol. 65, no. 10, ELSEVIER SCIENCE BV, 2013, pp. 1357–69, doi:10.1016/j.addr.2012.09.039.
[2] Cramer, Patrick. “AlphaFold2 And the Future of Structural Biology.†Nature Structural and Molecular Biology, vol. 28, no. 9, Nature Research, 2021, pp. 704–05, doi:10.1038/s41594-021-00650-1.
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Categories
Parameters
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