Coding
Part:BBa_K3841011:Design
Designed by: Emil Ørsted Christensen Group: iGEM21_DTU-Denmark (2021-09-02)
Particulate methane monooxygenase gamma subunit (PmoC)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 439
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 439
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 439
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 439
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 439
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used GeneWiz to codon optimize the sequence for expression in Komagataella phaffii. Additionally, we removed any BsaI and BsmBI sites within the coding sequence.
Source
10.2210/pdb3RGB/pdb