Composite

Part:BBa_K3838999:Design

Designed by: Siyang Yu   Group: iGEM21_SZU-China   (2021-08-30)


J-TES4


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 322
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 351
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 322
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 322
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

there are double terminator in this composite part in order to ensure termination efficiency.


Source

the tes4 gene is from Bacteroides thetaiotaomicron strain DSM 2079.

References