Composite
Part:BBa_K3838888:Design
Designed by: Siyang Yu Group: iGEM21_SZU-China (2021-08-30)
J-SOD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 394
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 394
Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 394
Illegal BglII site found at 437 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 394
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 394
Illegal NgoMIV site found at 598 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Some bacteria would express the SOD gene itself,which should be considered in the experiment.
Source
The SOD gene is from Lactobacillus casei strain Lc18.