Translational_Unit

Part:BBa_K3837004:Design

Designed by: In Geol Choi   Group: iGEM21_KUAS_Korea   (2021-09-19)


YF1/FixJ bicistronic operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 215
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 215
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 215
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 215
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 616
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 724
    Illegal NgoMIV site found at 1218
    Illegal NgoMIV site found at 1511
    Illegal NgoMIV site found at 1605
    Illegal AgeI site found at 258
    Illegal AgeI site found at 1386
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1275
    Illegal BsaI.rc site found at 157


Design Notes

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Source

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References