RNA
Prx lnhRNA
Part:BBa_K3835000:Design
Designed by: Wei Luen Huang Group: iGEM21_NTHU_Taiwan (2021-07-13)
It
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 145
Illegal EcoRI site found at 2016 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 145
Illegal EcoRI site found at 2016 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 145
Illegal EcoRI site found at 2016
Illegal BglII site found at 434
Illegal BglII site found at 1276
Illegal BglII site found at 1727 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 145
Illegal EcoRI site found at 2016 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 145
Illegal EcoRI site found at 2016 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The optimal length is 300-600nt, but some research indicates that the whole exon is more effective than part of the exon.
Source
It comes from pine wilt nematodes, Bursaphelenchus xylophilus. Prx, BXY_1749900, is a protein coding gene of 2-cysteine peroxiredoxin.