RNA
Prx lnhRNA

Part:BBa_K3835000:Design

Designed by: Wei Luen Huang   Group: iGEM21_NTHU_Taiwan   (2021-07-13)


It


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 145
    Illegal EcoRI site found at 2016
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 145
    Illegal EcoRI site found at 2016
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 145
    Illegal EcoRI site found at 2016
    Illegal BglII site found at 434
    Illegal BglII site found at 1276
    Illegal BglII site found at 1727
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 145
    Illegal EcoRI site found at 2016
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 145
    Illegal EcoRI site found at 2016
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The optimal length is 300-600nt, but some research indicates that the whole exon is more effective than part of the exon.

Source

It comes from pine wilt nematodes, Bursaphelenchus xylophilus. Prx, BXY_1749900, is a protein coding gene of 2-cysteine peroxiredoxin.

References