Coding
Scl2

Part:BBa_K3833000:Design

Designed by: Margaret Keymakh, Deniz Sinar   Group: iGEM21_Cornell   (2021-10-01)


Streptococcal Collagen-Like Protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 397
    Illegal PstI site found at 406
    Illegal PstI site found at 415
    Illegal PstI site found at 469
    Illegal NgoMIV site found at 517
    Illegal NgoMIV site found at 751
    Illegal NgoMIV site found at 1230
    Illegal AgeI site found at 1237
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to use Gibson Assembly to produce a recombinant plasmid, it is likely a good idea to add ~20bp of homology at both ends of the sequence with neighboring DNA sequences.


Source

https://www.uniprot.org/uniprot/Q8RLX7


References