Coding

Part:BBa_K3832010

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-10-21)


pykA (pyruvate kinase II)

This part encodes pyruvate kinase II (E.C. 2.7.1.40) in E.coli DH5a and K-12 substrain.

Reaction:

ATP + pyruvate = ADP + phosphoenolpyruvate

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 953
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 953
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 606
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 953
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None