Coding

Part:BBa_K3830013:Design

Designed by: Fang-Ti   Group: iGEM21_NCHU_Taichung   (2021-10-18)


celK is exoglycosidase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1687
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 545
    Illegal PstI site found at 1022
    Illegal PstI site found at 1907
    Illegal PstI site found at 1946
    Illegal PstI site found at 2312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1687
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 545
    Illegal PstI site found at 1022
    Illegal PstI site found at 1907
    Illegal PstI site found at 1946
    Illegal PstI site found at 2312
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1687
    Illegal EcoRI site found at 1755
    Illegal BamHI site found at 1368
    Illegal XhoI site found at 313
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1687
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 545
    Illegal PstI site found at 1022
    Illegal PstI site found at 1907
    Illegal PstI site found at 1946
    Illegal PstI site found at 2312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1687
    Illegal EcoRI site found at 1755
    Illegal PstI site found at 545
    Illegal PstI site found at 1022
    Illegal PstI site found at 1907
    Illegal PstI site found at 1946
    Illegal PstI site found at 2312
    Illegal AgeI site found at 1132
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1972


Design Notes

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Source

Clostridium thermocellum<I>

===References===