Composite

Part:BBa_K3827006:Design

Designed by: Livia Vieira   Group: iGEM21_British_Columbia   (2021-10-20)


pBAD - luxABG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1759
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 1587
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3114
    Illegal SapI site found at 961


Design Notes

Sequence taken from BioBrick BBa_K325909, luxABG and the annotated putative RBS

Source

Sequence taken from BioBrick BBa_K325909 https://parts.igem.org/cgi/partsdb/part_info.cgi?part_name=BBa_K325909

References