Composite

Part:BBa_K3826014

Designed by: Lucas Müller   Group: iGEM21_Duesseldorf   (2021-10-21)


CAP160 Chromo HSP

This reporter construct consists of the CAP160 promoter, the ChromoGFP reporter and the Arabidopsis thaliana HSP terminator. Chromoprotein (amilGFP) is a yellow chromoprotein from the coral Acropora millepora. CAP160 is induced during periods of drought stress. Claudia Kaye et al. (https://academic.oup.com/plphys/article/116/4/1367/6085590)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 577
    Illegal XbaI site found at 893
    Illegal XbaI site found at 1209
    Illegal XbaI site found at 1679
    Illegal XbaI site found at 2008
    Illegal SpeI site found at 1316
    Illegal PstI site found at 1039
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 577
    Illegal SpeI site found at 1316
    Illegal PstI site found at 1039
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 577
    Illegal BglII site found at 364
    Illegal BglII site found at 1523
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 577
    Illegal XbaI site found at 893
    Illegal XbaI site found at 1209
    Illegal XbaI site found at 1679
    Illegal XbaI site found at 2008
    Illegal SpeI site found at 1316
    Illegal PstI site found at 1039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 577
    Illegal XbaI site found at 893
    Illegal XbaI site found at 1209
    Illegal XbaI site found at 1679
    Illegal XbaI site found at 2008
    Illegal SpeI site found at 1316
    Illegal PstI site found at 1039
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None