Composite

Part:BBa_K3826011:Design

Designed by: Lucas Müller   Group: iGEM21_Duesseldorf   (2021-10-21)


PR1 promoter RUBY HSP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2041
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2041
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1927
    Illegal BglII site found at 5785
    Illegal BamHI site found at 4434
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2041
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2041
    Illegal NgoMIV site found at 2370
    Illegal NgoMIV site found at 2949
    Illegal NgoMIV site found at 4662
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Activation of a truncated PR-1 promoter by endogenous enhancers in transgenic plants, Andrea Beilmann et al. 1992 RUBY as commonly used reporter, (Yubing He et al. 2020), BBa_K3900028 HSP: Terminator in Arabidopsis thaliana (S Nagaya et al,2009)


Source

Activation of a truncated PR-1 promoter by endogenous enhancers in transgenic plants, Andrea Beilmann et al. 1992 RUBY as commonly used reporter, (Yubing He et al. 2020), BBa_K3900028 HSP: Terminator in Arabidopsis thaliana (S Nagaya et al,2009)


References