Composite
Part:BBa_K3823014:Design
Designed by: Yiying Zhang Group: iGEM21_Tongji_China (2021-10-02)
T7-SQR-J23110-SDO-AprBA-SAT
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 190
Illegal XbaI site found at 47
Illegal XbaI site found at 3581
Illegal SpeI site found at 2054
Illegal PstI site found at 1771
Illegal PstI site found at 2983 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 190
Illegal NheI site found at 151
Illegal NheI site found at 1722
Illegal NheI site found at 1745
Illegal NheI site found at 2568
Illegal NheI site found at 2591
Illegal NheI site found at 3473
Illegal NheI site found at 3496
Illegal SpeI site found at 2054
Illegal PstI site found at 1771
Illegal PstI site found at 2983
Illegal NotI site found at 1525 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 190
Illegal BglII site found at 2254
Illegal BamHI site found at 184
Illegal BamHI site found at 3786
Illegal BamHI site found at 5241
Illegal XhoI site found at 1534 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 190
Illegal XbaI site found at 47
Illegal XbaI site found at 3581
Illegal SpeI site found at 2054
Illegal PstI site found at 1771
Illegal PstI site found at 2983 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 190
Illegal XbaI site found at 47
Illegal XbaI site found at 3581
Illegal SpeI site found at 2054
Illegal PstI site found at 1771
Illegal PstI site found at 2983
Illegal NgoMIV site found at 255
Illegal NgoMIV site found at 679
Illegal NgoMIV site found at 1980
Illegal NgoMIV site found at 4260
Illegal NgoMIV site found at 4431
Illegal NgoMIV site found at 5114
Illegal AgeI site found at 1174
Illegal AgeI site found at 2001
Illegal AgeI site found at 2794 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
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