Part:BBa_K3819128

Aspergillus niger lipase

Microbial lipases catalyze the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids, is widely distributed in nature. It is a class of enzymes that can carry out ester hydrolysis or ester synthesis at the oil-water interface or in water-insoluble systems. Apart from their natural substrates, lipases catalyze the chemoselective, the enantioselective and the regioselective hydrolysis and synthesis of a broad range of non-natural esters, and are widely used in industry. Acid lipase is a type of lipase that can stably and efficiently hydrolyze oil in an acidic environment. It can not only assist cellulase and xylanase in deinking during deinking, but also effectively treat the deinking after deinking. Sewage. Therefore, it has a good application prospect in deinking.treatment after deinking. The Aspergillus in the filamentous fungus is one of the important sources for obtaining high-performance acid lipase. We chose the lipase from Aspergillus niger F044 (https://www.ncbi.nlm.nih.gov/nuccore/ 109677002/), NCBI accession number is: DQ647700.1, its optimum pH is 5.0, it has high activity at 60℃, which is helpful for deinking and sewage treatment.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]