Composite

Part:BBa_K3819099:Design

Designed by: Liyan Wu   Group: iGEM21_UESTC-China   (2021-10-05)


EG1--(GGGGS)3 linker -linker-CcsDockerin--His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.


Source

This part contains cellulase EG1,2 (GGGGS) 3 linkers, CCsDockerin for connecting cellulase and backbone, and His-tag for verification.

References