Composite

Part:BBa_K3819088:Design

Designed by: Liyan Wu   Group: iGEM21_UESTC-China   (2021-09-28)

XynB - GS linker - CtDockerin - His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.


Source

1.XynB(K3819062) 2.(GGGGS)3 linker(K3819072) 3.CtDockerin(K3819015) 4.His-tag(K3819051)

References