Composite

Part:BBa_K3819077:Design

Designed by: Liyan Wu   Group: iGEM21_UESTC-China   (2021-10-05)


EGL7 plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization and the restriction site optimization. And we add linker to connect the cellulase and dockerin so that the cellulase bind with the cohesin on the scaffold and anchor to the yeast cell surface finally.


Source

1 GAP promoter BBa_K3819009, (FN392320.1, Pichia pastoris GS115 chromosome 2, complete sequence) 2 EGL7(BBa_K3819003) 3 linker (GGGGS)3 （GGTGGTGGTGGTAGTGGTGGTGGTGGTAGTGGTGGTGGTGGTAGT）(BBa_K3819072) 4 CcsDockerin (BBa_K3819066) 5 His tag (6xHis-tag)(BBa_K3819051)

References