Coding

Part:BBa_K3819016:Design

Designed by: Luo SongWen   Group: iGEM21_UESTC-China   (2021-09-28)


(GGGGS) 2 linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.


Source

Liu T, Meng X, Chen J, Fang Q, Wang J. Smt3, a homologue of yeast SUMO, contributes to asexual development, environmental adaptation, and host infection of a filamentous entomopathogen. Fungal Biol. 2020 Nov;124(11):924-931. doi: 10.1016/j.funbio.2020.07.010. Epub 2020 Aug 8. PMID: 33059844.


References