Coding
Part:BBa_K3819016:Design
Designed by: Luo SongWen Group: iGEM21_UESTC-China (2021-09-28)
(GGGGS) 2 linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized to improve expression in yeast and the restriction site optimized to connect a linker directly.
Source
Liu T, Meng X, Chen J, Fang Q, Wang J. Smt3, a homologue of yeast SUMO, contributes to asexual development, environmental adaptation, and host infection of a filamentous entomopathogen. Fungal Biol. 2020 Nov;124(11):924-931. doi: 10.1016/j.funbio.2020.07.010. Epub 2020 Aug 8. PMID: 33059844.