Regulatory

Part:BBa_K3819009

Designed by: Yuwei Hu   Group: iGEM21_UESTC-China   (2021-09-25)


Gap Promoter

This promoter used in recombinant protein production is derived from glyceraldehyde 3-phosphate dehydrogenase (GAP). The GAP gene is constitutively expressed in cells grown on glucose, glycerol, and methanol. In recombinant protein production under the control of pGAP, biomass increases, while protein is synthesized at the same time. The level of GAP promoter activity is two-thirds in glycerol medium and one-third in methanol medium when compared to glucose medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None