Other

Part:BBa_K3819005:Design

Designed by: Luo SongWen   Group: iGEM21_UESTC-China   (2021-09-25)


SMT3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have adopted strategies such as codon optimization to change the restriction site in order to be compatible with RFC.


Source

Gene ID: 852122,https://www.ncbi.nlm.nih.gov/gene/852122 Reference: [1]Liu T, Meng X, Chen J, Fang Q, Wang J. Smt3, a homologue of yeast SUMO, contributes to asexual development, environmental adaptation, and host infection of a filamentous entomopathogen. Fungal Biol. 2020 Nov;124(11):924-931.doi: 10.1016/j.funbio.2020.07.010. Epub 2020 Aug 8. PMID: 33059844 [2]Esteras M, Liu IC, Snijders AP, Jarmuz A, Aragon L. Identification of SUMO conjugation sites in the budding yeast proteome. Microb Cell. 2017 Oct 2;4(10):331-341. doi: 10.15698/mic2017.10.593. PMID: 29082231; PMCID: PMC5657824.

References