Composite

Part:BBa_K3817014:Design

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


Cdh pathway


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 350
    Illegal NheI site found at 133
    Illegal NheI site found at 2074
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 350
    Illegal BglII site found at 239
    Illegal XhoI site found at 1039
    Illegal XhoI site found at 2308
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 350
    Illegal XbaI site found at 47
    Illegal PstI site found at 2982
    Illegal PstI site found at 3535
    Illegal AgeI site found at 777
    Illegal AgeI site found at 1269
    Illegal AgeI site found at 1437
    Illegal AgeI site found at 1672
    Illegal AgeI site found at 1912
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We need to consider where each basic part is placed to ensure that it performs the transcription and translation processes most efficiently, which needs to satisfy the principles of biological design. In addition, because of the large molecular weight of the enzyme, we needed to consider whether it could be secreted or whether it was present in the inclusion bodies, which had a significant impact on our subsequent experiments.


Source

It comes from Pseudomonas sp. CBB1 and E.coli.

References