Coding

Part:BBa_K3817005:Design

Designed by: Sun Ji   Group: iGEM21_UCAS-China   (2021-10-01)


Ptrc temp-sensitive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 78
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 78
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 78
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 78
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The temperature control promoter can help us to realize the idea of conditionally controlling whether the biosafety module is turned on or not, and preventing gene leakage by regulation.


Source

It comes from the E.coli. plasmids pKBi.

References