Composite

Part:BBa_K3814075

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)


Cluster 7

We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli.

To do this, we have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome. Thus, rather than inserting each of the 23 genes individually, we can transform them in clusters of genes.

We determined these clusters by assessing the biological function of each gene and through k-means clustering, and one such is shown here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 716
    Illegal NheI site found at 739
    Illegal NheI site found at 1970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2805
    Illegal XhoI site found at 75
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 420
    Illegal AgeI site found at 1106
    Illegal AgeI site found at 2271
  • 1000
    COMPATIBLE WITH RFC[1000]


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