Part:BBa_K3814067

fliK Landing Pad (34%)

This is the fliK Landing Pad (34%), an integral part of the USYD 2021 team's project. We aim to produce naturally transformable (NT) E. coli by inserting the NT genes of another species into it. We have chosen 23 genes from Acinetobacter baylyi and have planned to insert them into the fliK gene in the E. coli. We have devised a novel recombineering strategy that allows for homologous recombination to insert large amounts of DNA sequentially into the chromosome, and this 'landing pad' is the beginning of this process. It provides all the foundations for the 23 A. baylyi genes to be inserted into the chromosome and be expressed.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1087
  Illegal NheI site found at 1110
  Illegal NheI site found at 2383
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2529
  Illegal XhoI site found at 1219
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2555
  Illegal NgoMIV site found at 2923
  Illegal NgoMIV site found at 3083
  Illegal AgeI site found at 1513
  
• 1000
  COMPATIBLE WITH RFC[1000]