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Part:BBa_K3814050

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)


fliK downstream homology region

This 50bp section of DNA is from the fliK gene that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome.

This is a region of the fliK gene, and gene clusters will be recombineered into it in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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