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Part:BBa_K3814028

Designed by: Simon Tang   Group: iGEM21_Sydney_Australia   (2021-10-01)


5' Homology Arm for Cluster 6

This 50bp section of DNA is random DNA that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome. This and all other homology arms listed in the parts registry can be used by future teams. This homology arm have been designed to minimise self-dimerisation and hairpin formations, as in USYD 2021's project, it will also exist as a separate primer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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