![](https://parts.igem.org/images/partbypart/icon_plasmid_backbone.png)
Plasmid_Backbone
Part:BBa_K3809015:Design
Designed by: Francisco Javier Castañeda Villagran Group: iGEM21_TecCEM (2021-10-19)
Ori and ampR from pUC with modified MCS for BB Assembly
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Illegal suffix found at 1919
Illegal EcoRI site found at 1840 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1840
Illegal SpeI site found at 1920
Illegal PstI site found at 1934
Illegal NotI site found at 1846
Illegal NotI site found at 1927 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1840
Illegal BamHI site found at 1889 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Illegal suffix found at 1920
Illegal EcoRI site found at 1840 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 1840
Plasmid lacks a suffix.
Illegal SpeI site found at 1920
Illegal PstI site found at 1934 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 866
Design Notes
We added two restriction sites for HindIII and NcoI so it could be easier for us to assembly this part with a katE gene we designed.
Source
We retrieved the elements from plasmid pUC19 from Addgene.