Plasmid_Backbone

Part:BBa_K3809015:Design

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-10-19)


Ori and ampR from pUC with modified MCS for BB Assembly


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Illegal suffix found at 1919
    Illegal EcoRI site found at 1840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1840
    Illegal SpeI site found at 1920
    Illegal PstI site found at 1934
    Illegal NotI site found at 1846
    Illegal NotI site found at 1927
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1840
    Illegal BamHI site found at 1889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Illegal suffix found at 1920
    Illegal EcoRI site found at 1840
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1840
    Plasmid lacks a suffix.
    Illegal SpeI site found at 1920
    Illegal PstI site found at 1934
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 866


Design Notes

We added two restriction sites for HindIII and NcoI so it could be easier for us to assembly this part with a katE gene we designed.


Source

We retrieved the elements from plasmid pUC19 from Addgene.

References