Composite

Part:BBa_K3809003:Design

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-08-13)


katE regulated by BBa_J23100


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1962
    Illegal BamHI site found at 1872
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to modify some codons in order to meet the iGEM Biobrick Standards. We also had to modify some nucleotides to synthetize the sequence with IDT.


Source

The RBS comes from BBa_B0030, the spacer comes from BBa_B0040, the promoter comes from BBa_J23100 and terminator from BBa_B0010. The coding sequence for the katE gene comes from NCBI GenBank: M55161.1. It is the sequence from E.coli catalase HPII (katE) gene, complete cds. However, some codons were optimized in order to make it compatible with Biobrick standards.

References