![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3809002:Design
Designed by: Francisco Javier Castañeda Villagran Group: iGEM21_TecCEM (2021-08-13)
This part contains katE with a promoter, terminator and RBS.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2354
Illegal NheI site found at 2377 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1842
Illegal BamHI site found at 1752 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 111
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to modify some codons in order to meet the iGEM Biobrick Standards. We also had to modify some nucleotides to synthetize the sequence with IDT.
Source
The RBS comes from BBa_B0030, the spacer comes from BBa_B0040, the promoter comes from BBa_J23100 and terminator from BBa_B0010. The coding sequence for the katE gene comes from NCBI GenBank: M55161.1. It is the sequence from E.coli catalase HPII (katE) gene, complete cds. However, some codons were optimized in order to make it compatible with Biobrick standards.