Coding

Part:BBa_K3809002

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-08-13)


This part contains katE with a promoter, terminator and RBS.

This Biobrick contains a katE gene with an RBS (BBa_B0030), spacer (BBa_B0040), terminator (BBa_B0010) and promoter (BBa_J23100). It was designed to be expressed as part of a plasmid, to act as a selection cassette for a modified strain of E. coli without genes involved in the methabolic pathways of glycerol and peroxide. It may be also used for other purposes not stated here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2354
    Illegal NheI site found at 2377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1842
    Illegal BamHI site found at 1752
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 111
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None