![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
katE
Part:BBa_K3809000:Design
Designed by: Francisco Javier Castañeda Villagran Group: iGEM21_TecCEM (2021-08-13)
This part contains the coding region of E. coli catalase katE gene.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 92
Illegal NheI site found at 115 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We had to modify some codons in order to meet the iGEM Biobrick Standards. We also had to modify some nucleotides to synthetize the sequence with IDT.
Source
The RBS comes from BBa_B0030, the spacer comes from BBa_B0040, the promoter comes from BBa_J23100 and terminator from BBa_B0010. The coding sequence for the katE gene comes from NCBI GenBank: M55161.1. It is the sequence from E.coli catalase HPII (katE) gene, complete cds. However, some codons were optimized in order to make it compatible with Biobrick standards.