Composite

Part:BBa_K3807018:Design

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


Riboswitch + selection device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2516
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2516
    Illegal NheI site found at 1205
    Illegal NheI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2516
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1587
    Illegal BamHI site found at 2486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2516
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2516
    Illegal NgoMIV site found at 1613
    Illegal NgoMIV site found at 1981
    Illegal NgoMIV site found at 2141
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2672
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Designed to be a complete expression device to examine the expression level of TetA-sfGFP (reporter protein linked to the expression level) controlled by an inducible promoter and theophylline riboswitch.

Source

Gibson Assembly of IDT gene fragments.

References

Muranaka et al, 2009