Coding
Part:BBa_K3807004:Design
Designed by: Sarah Vorsselmans Group: iGEM21_KU_Leuven (2021-10-01)
ΔsfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 151
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutation introduced at amino acid position 93 Y->X (nucleotides 279 G->T) by PCR
Source
Site-directed mutagenesis of a sfGFP cassette