Coding

Part:BBa_K3807004:Design

Designed by: Sarah Vorsselmans   Group: iGEM21_KU_Leuven   (2021-10-01)


ΔsfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation introduced at amino acid position 93 Y->X (nucleotides 279 G->T) by PCR

Source

Site-directed mutagenesis of a sfGFP cassette

References