Composite

Part:BBa_K3806014:Design

Designed by: Laura Sierra Heras   Group: iGEM21_TUDelft   (2021-09-30)


Theophylline-binding aptazyme regulating lacZ expression (cRBS). With T7 promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2321
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part constitutes a biosensing unit based on a theophylline aptazyme sequence flanked by a ribosomal binding site and ribosomal binding site complementary sequence (BBa_K3806010). A codon optimized lacZ (BBa_K3806012) acts as a reporter gene. The construct is preceded by a T7 promoter (BBa_K3633015) for transcription by T7 bacteriophage RNA polymerase.