Part:BBa_K3798033

Fusion protein sequence comprised with two CBD domain and one streptavidin domain, B.subtilis optimi This part is modified from the previous part BBa_K1499004 https://parts.igem.org/Part:BBa_K1499004. From this part, we optimized the Condon usage of this protein for Bacillus subtilis. CBD-SA is the linker module which connects cellulose and aptamer. It is a fusion protein composed of two cellulose-binding-domain and one Streptavidin domain that is produced by engineered Bacillus subtilis. The actual structure of this linker module refers to CBD-SA-CBD. We wanted to use system with B. subtilis to optimize our production of CBD-SA. But, the plasmids of CBD-SA were not transformed successfully into competent cells of B. subtilis.

In those discussions, however, our team have realized some of the errors that we made, namely, the wrong shaking frequency of the shaker that we set for 200rpm instead of 90 rpm, resulting in the death of the competent cells. Although we have formed plans of solving this problem, our B.subtilis transformation experiment cannot be continue due to the limited time and the used out competent cells.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]