Part:BBa_K3798011

ssDNA, an aptamer for Ag+ ion

Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K37980011 is a Ag+ aptamer obtained from a previous paper[1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Binding efficiency and stability

Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers.

Aptamer 10 shows no binding efficacy and was not stable in performance. Therefore, this aptamer is discarded and was not subjected for further examination.

Reference

1. Saran, R., Yao, L., Hoang, P., & Liu, J. (2018). Folding of the silver aptamer in a DNAzyme probed by 2-aminopurine fluorescence. Biochimie, 145, 145-150. doi: 10.1016/j.biochi.2017.07.001