Part:BBa_K3798010

ssDNA, an aptamer for Ag+ ion

Silver is one of the most wasted precious metals that can be found in effluents, streams and even food. As our project is to develop a precious metal recycling platform, we chose Ag+ as the demonstration ion of this phase of the project. Aptamers are oligonucleotides that can bind targeted molecules with its secondary structures, in our project, Ag+ ion. BBa_K37980010 is a Ag+ aptamer obtained from a previous paper[1].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Binding efficiency and stability

Fig.1 Remaining Ag+ ion concentration of the solution after treated by different types of aptamers.

Aptamer 9 shows a binding efficacy of only 36%, and was not stable in performance. Therefore, this aptamer is discarded and was not subjected for further examination.

Reference

1. Saran, R., Yao, L., Hoang, P., & Liu, J. (2018). Folding of the silver aptamer in a DNAzyme probed by 2-aminopurine fluorescence. Biochimie, 145, 145-150. doi: 10.1016/j.biochi.2017.07.001