Composite
Part:BBa_K3796207:Design
Designed by: Yang Feiyue Group: iGEM21_CAU_China (2021-09-24)
Patp2-rbs-gfp-terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 14
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 14
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 14
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 14
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 14
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added a short sequence CCTGCAGG before bjGFP to ensure proper distance between rbs and bjGFP, leading to higher translation efficiency.
Source
The promoter Patp2 comes from Corynebacterium glutamicum ATCC 13032.
The rbs sequence information is from BBa_K2963006 and the sequence itself is synthetic.
The bjGFP is originally chemical syhthesized and we amplified this sequence from the plasmid pRJPaph-bjGFP.
The rrnB terminators sequence information is from BBa_K3568009 and the sequences for rrnB T1 and T2 are carried by the plasmid pXMJ19.