Coding
Part:BBa_K3796202:Design
Designed by: Yiwei Wang Group: iGEM21_CAU_China (2021-09-07)
Phosphoenolpyruvate carboxylase (ppc) from C.glutamicum ATCC 13032
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal BglII site found at 1617
Illegal BglII site found at 2081
Illegal BamHI site found at 2179
Illegal BamHI site found at 2194
Illegal XhoI site found at 871
Illegal XhoI site found at 1516 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1099
Illegal EcoRI site found at 1540
Illegal PstI site found at 1684
Illegal PstI site found at 1783
Illegal PstI site found at 2167
Illegal NgoMIV site found at 1627 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2001
Illegal BsaI.rc site found at 767
Illegal SapI.rc site found at 259
Design Notes
high GC
Source
C.glutamicum ATCC 13032 genome(Genebank:BA000036.3)