Composite

Part:BBa_K3796008:Design

Designed by: Li Mingyang   Group: iGEM21_CAU_China   (2021-09-04)


capABC for gamma-PGA synthesis


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2046
    Illegal XbaI site found at 2341
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2046
    Illegal NheI site found at 2487
    Illegal NheI site found at 2491
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2046
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2046
    Illegal XbaI site found at 2341
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2046
    Illegal XbaI site found at 2341
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
    Illegal NgoMIV site found at 2679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These parts were ligated by Gibson assembly, transferred into plasmid pXMJ19 for validation, and subsequently transferred into plasmid pK18mobsacB for introduction into the genome.


Source

Coding sequences are from Bacillus subtilis str.168 genome and the promoter is from Corynebacterium glutamicum ATCC13032 genome. The T7 terminator sequence information is from BBa_K2598024 and the sequence itself is synthetic.

References