Part:BBa_K3796003

capB from Bacillus subtilis str.168

Catalytic synthesis of γ-polyglutamic acid from L-glutamic acid, also known as pgsB.

Usage and Biology

BBa_K3796003(capB) is partially of the gene cluster to synthesize γ-PGA, in which capA, capB and capC genes must be used altogether. When they are expressed together, they may be able to synthesize polyglutamic acid. Three genes can be placed downstream of the same promoter and upstream of the same terminator, or placed separately.

capABC gene is a gene cluster widely contained in Bacillus sp., which is also named pgsBCA gene cluster. capABC gene cluster can be used to synthesize γ-Polyglutamic acid(γ-PGA) with L-type or D-type glutamate as raw material. Gene capA has catalytic activity and capB has transport activity. The proteins encoded by these three genes work together and can synthesize γ- PGA and transport it to vitro in the form of capsule or free.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 737
  Illegal XbaI site found at 1032
  Illegal PstI site found at 382
  Illegal PstI site found at 1013
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 737
  Illegal PstI site found at 382
  Illegal PstI site found at 1013
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 737
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 737
  Illegal XbaI site found at 1032
  Illegal PstI site found at 382
  Illegal PstI site found at 1013
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 737
  Illegal XbaI site found at 1032
  Illegal PstI site found at 382
  Illegal PstI site found at 1013
  
• 1000
  COMPATIBLE WITH RFC[1000]