Coding

Part:BBa_K3795001:Design

Designed by: Isis Botelho Nunes da Silva   Group: iGEM21_Rio_UFRJ_Brazil   (2021-10-11)


DME-BR: Multi-epitope protein for anti Dengue antibodies detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 416
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 276
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is codon-optimized for expression in Escherichia coli. Besides, restriction sites for Biobrick Standard Assembly (EcoRI, PstI, XbaI, SpeI) and also BamHI and NocI were removed.


Source

It was designed by the iGEM 2021 team Rio_UFRJ_Brazil through the combination of promising Dengue epitopes in order to minimize cross-reaction with anti-Zika antibodies. After the design, it was synthesized by Genscript.

References