Composite

Part:BBa_K3794001:Design

Designed by: Harshraj Bumia   Group: iGEM21_KCL_UK   (2021-09-30)


PVFP-5 Composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This translational unit was synthesised by IDT as a composite part. A LacI regulator (BBa_R0010) was added upstream and an N-terminal 6xHis tag + TEV cleavage site were added to allow for protein expression in E.coli and protein purification using a Ni-NTA column respectively. This DNA sequence has been codon optimised for expression in E.coli K12.


Source

This translational unit for PVFP-5 was synthesised by IDT directly. The coding sequence for PVFP-5 protein was obtained from UniProtKB U5Y3S6, a removal of the signal peptide was conducted alongside a codon optimisation for expression in E.coli K12.

References