DNA

Part:BBa_K3791014:Design

Designed by: Laura S�nchez Ruiz, Auba Fuster Pal�   Group: iGEM21_UPF_Barcelona   (2021-10-10)


gRNA Spectinomycin construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The repeat sequence must be placed before the spacer sequence, that is, in the 5’ extreme. It is also required to have a length between 18 and 24 nucleotides, in this case adjusting it to a consensus G-C content of approximately 50%.

Source

The repeat and the T7 promoter sequences were extracted from the parts registry (BBa_K2927006) (BBa_K1614000), whereas the spacer one was designed with the constraint that it had to be complementary to the sequence of the spectinomycin-resistance gene aiming to be detected (as explained in its own part page: BBa_K3791004).