DNA

Part:BBa_K3791008

Designed by: Laura Sánchez Ruiz, Auba Fuster Palŕ   Group: iGEM21_UPF_Barcelona   (2021-10-09)


gRNA Kanamycin

Here we post the full guide RNA that allows for targeting a specific kanamycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein (BBa_K2927006), and a spacer sequence which is specific for the desired gene to target (BBa_K3791003).


Usage, Biology and Characterization

As this part is a simplification of our final construct, usage, biology and characterization is explained there: BBa_K3791013


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/crispr-cas/diagnosis
//function/crispr/grna
Parameters
None